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JoVE Journal Neuroscience
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
 

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat

Article DOI: 10.3791/4079-v • 12:45 min • July 19th, 2012
July 19th, 2012 •
1, 2, 3, 1,4
1Helen Wills Neuroscience Institute, University of California Berkeley, 2Office of Laboratory Animal Care, University of California Berkeley, 3McGovern Institute for Brain Research & The Department of Brain and Cognitive Science, Massachusetts Institute of Technology, 4Integrative Biology Department, University of California Berkeley

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Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.

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Stereotaxic Surgery Excitotoxic Lesion Specific Brain Areas Adult Rat Localized Ablation Electrolytic Lesions Inducible Genetic Techniques Cell-type Specific Promoters Stereotaxic Infusion Excitotoxin N-methyl-D-aspartate (NMDA) Basolateral Amygdala Complex
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