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反向遗传学感染小鼠诺沃克病毒介导的恢复
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
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反向遗传学感染小鼠诺沃克病毒介导的恢复

DOI:
10.3791/4145-v

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13:48 min

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June 24, 2012

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, , , ,
1Section of Virology,Imperial College London

Capítulos

  • 00:05Título
  • 01:34Procedure Overview
  • 03:16RNA Transcription and Capping for the Recovery of Infectious MNV
  • 06:27MNV Recovery by RNA Electroporation into Raw Cells
  • 08:25Recovery by Lipofection into BSR-T7 Cells
  • 10:07Direct Recovery of Infectious MNV from cDNA in Cells Expressing T7 RNA Polymerase
  • 12:20Representative Results

Summary

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Tadução automática

诺如病毒肠胃炎尚未分子技术是表征的主要原因仍然是相对较新的。在这里，我们报告两个不同的反向遗传学方法对小鼠诺沃克病毒（MNV），本属的唯一成员，可在细胞培养繁殖的高效回收。

Tags

Reverse GeneticsRecoveryInfectiousMurine NorovirusHuman NorovirusesGastroenteritisOutbreaksHospitalsAntiviral ApproachesCell CulturePropagationGenomic RNA MoleculesOpen Reading Frames (ORFs)Non-structural ProteinsCapsid ProteinsVirulence Factor (VF1)

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