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Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
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JoVE Journal Medicina
Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

DOI:
10.3791/4221-v

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11:31 min

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October 31, 2012

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, , , , , , , ,
1Department of Surgery,Stanford University , 2Department of Surgery,Duke University , 3Department of Surgery,Saint Joseph Mercy Hospital, 4School of Medicine,University of California, San Francisco , 5School of Dentistry,University of California, Los Angeles

Capítulos

  • 00:05Título
  • 01:38Cell Isolation and Expansion
  • 05:10Cell Seeding, Creating Calvarial Defects and in vivo Implantation
  • 07:47Quantification of Osteoid Formation
  • 09:07Results: Repair of Critical-size Defect by Human Adipose-derived Stromal Cells
  • 10:50Conclusion

Summary

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This protocol describes the isolation of adipose-derived stromal cells from lipoaspirate and the creation of a 4 mm critical-sized calvarial defect to evaluate skeletal regeneration.

Tags

RepairCritical-sized Calvarial DefectAdipose-derived Stromal CellsLipoaspirateCraniofacial Skeletal RepairCell-based ApproachStem CellsOsteogenic PotentialScaffolding BiomaterialsCellular DeliveryOsteoconductiveHydroxyapatite-coated Poly(lactic-co-glycolic Acid)HA-PLGA ScaffoldOsseous RegenerationTranslational ApproachesProgenitor Cell

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