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The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
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Capítulos
- 00:05Título
- 01:22Insect Rearing and Selection of Larvae for Infection
- 04:06Bacterial and Cry Toxin Preparation
- 06:35Insect Intrahaemocoelic Injection
- 07:55Insect Force Feeding (Ingestion)
- 09:24Recording Insect Mortality
- 10:21Analysis of Insect Mortality: Representative Results
- 12:16Conclusion
Oral and intra haemocolic infection of larvae of the greater wax moth Galleria mellonella is described. This insect can be used to study virulence factors of entomopathogenic as well as mammalian opportunistic bacteria. Rearing of the insects, methods of infection and examples of in vivo analysis are described.
Tags
Insect Galleria MellonellaBacterial PathogenesisInfection ModelAnimal ModelInsects As Infection ModelsNon-vertebrate Model HostsAntimicrobial DefensesInnate Immune SystemPhagocytosisEncapsulationHumoral ResponsesLysozymeAntibacterial PeptidesEpithelial CellsLarval MidgutsMammalian Digestive SystemsMicrobial Virulence FactorsGreater Wax Moth Galleria Mellonella
