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E4orf4诱导的细胞死亡的击倒效果的基因表达和测量条件击倒细胞系的制备
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Biologia
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JoVE Journal Biologia
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
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E4orf4诱导的细胞死亡的击倒效果的基因表达和测量条件击倒细胞系的制备

DOI:
10.3791/4442-v

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13:54 min

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October 21, 2012

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, ,
1Department of Molecular Microbiology, Faculty of Medicine,Technion – Israel Institute of Technology

Capítulos

  • 00:05Título
  • 01:50Generation of Inducible Cell Lines
  • 06:19Induction of Knockdown and Transfection
  • 08:08DAPI Assay in Transfected Cells
  • 10:59Results: Detection of E4orf4-induced Cell Death in Inducible Cell Lines
  • 13:06Conclusion

Summary

Tadução automática

Tadução automática

ACF染色质重塑因子E4orf4诱导的细胞死亡的贡献进行了测量。该协议包括在多西环素治疗诱导有条件击倒的ACF的亚基ACF1和SNF2h的细胞克隆的选择，使用的DAPI检测测量E4orf4-诱导的细胞死亡的诱导的细胞系。

Tags

Conditional KnockdownGene ExpressionCell LinesACF Chromatin Remodeling FactorDoxycycline-inducible PromoterNeomycin Resistance GeneShRNA ConstructsAcf1SNF2hE4orf4 ProteinCell Death

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