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Capítulos
- 00:05Título
- 01:50Exposing the Kidney in a Munich Wistar Rat for Intravital 2-Photon Imaging
- 04:10Acquiring Images to Quantify Renal Permeability of Albumin
- 06:02Calculating Glomerular Sieving Coefficient for Fluorescent Albumin from 3D Volumes
- 08:21Glomerular Permeability Results
- 10:34Conclusion
A technique utilizing high resolution intavital 2-photon microscopy to directly visualize and quantify gloemrular filtration in surface glomeruli. This method allows for direct determination of permeability characteristics of macromolecules in both normal and diseased states.
Tags
2-photon MicroscopyGlomerular PermeabilityFluorescent MacromoleculesMunich Wistar RatsKidney DiseasesUrinary LossSerum AlbuminPodocytesVascular Endothelial CellsBasement MembraneGlomerular Filtration BarrierProximal Tubule CellsMicropunctureDextran PolymersBowman’s SpaceAlbumin ReabsorptionTubular TranscytosisProteinuric Renal Diseases
