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Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
 

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Article DOI: 10.3791/50156-v • 10:34 min • February 1st, 2013
February 1st, 2013 •
1,2, 1,2
1Department of Obstetrics, Gynecology & Women's Health, Masonic Cancer Center, University of Minnesota, Minneapolis, 2Department of Genetics, Cell Biology & Development, Center for Genome Engineering, University of Minnesota, Minneapolis

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Resumo
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A method of identifying unknown drivers of carcinogenesis using an unbiased approach is described. The method uses the Sleeping Beauty transposon as a random mutagen directed to specific tissues. Genomic mapping of transposon insertions that drive tumor formation identifies novel oncogenes and tumor suppressor genes

Tags

Sleeping Beauty Transposon Insertions Solid Tumors Linker-mediated PCR Genomic Analysis Proteomic Analysis Transcriptomic Analysis Epigenomic Analysis Cancer Genome Genetic Drivers Of Cancer Passenger Anomalies Genetic Heterogeneity Unbiased Forward Genetic Screen Mice Transposon System Transposase Enzyme Conditional Transposase Allele Cre Recombinase
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