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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
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Capítulos
- 00:05Título
- 01:28Prepare Optic Cable
- 02:36Screen for Transgene Expression
- 03:27Larvae Mounting and Optical Stimulation Preparation
- 05:02Activate Single Neurons Using a 473 nm Laser
- 05:59Results: Optogenetic Activiation of Zebrafish Somatosensory Neurons Using ChEF-tdTomato
- 06:56Conclusion
Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.
