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Neuroscience

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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
 

Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

Article DOI: 10.3791/50184-v 07:25 min January 31st, 2013
January 31st, 2013

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Summary

Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.

Tags

Keywords: Optogenetics Zebrafish Somatosensory Neurons ChEF-tdTomato Neural Circuit Channelrhodopsin Behavior Transgene CREST3 Confocal Microscopy 473 Nm Laser High-speed Video
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