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Journal / Neuroscience / A Method for High Fidelity Optogenetic Control of …
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Neuroscience

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JoVE Journal Neuroscience
A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo
 

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

Article DOI: 10.3791/50291-v • 13:44 min • September 2nd, 2013
September 2nd, 2013 •
*1,2, *1,2, 1,2
1Institute for Behavioral Genetics, University of Colorado Boulder, 2Department of Psychology and Neuroscience, University of Colorado Boulder
* These authors contributed equally

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Summary
Automatic Translation

The recent development of neuroscience tools that combine genetics and optics, termed "optogenetics", enables control over neural circuit activity with an unprecedented level of spatial and temporal resolution. Here we provide a protocol for integrating in vivo recording with optogenetic manipulation of genetically-defined subsets of prefrontal cortical and subicular pyramidal neurons.

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Keywords: Optogenetics Neural Circuit Channelrhodopsin-2 Halorhodopsin Adeno-associated Virus Dorsal Subiculum Prelimbic Region Prefrontal Cortex Pyramidal Neuron In Vivo Electrical Activity Light Delivery Electrical Recording
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