Journal
/
Neuroscience
/
Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

DOI:
10.3791/53628-v

•

11:13 min

•

April 05, 2016

•

, ,
1Hertie Institute for Clinical Brain Research and Werner Reichardt Centre for Integrative Neuroscience,University of Tuebingen, 2Max Planck Florida Institute for Neuroscience

Chapters

  • 00:05Title
  • 01:19Preparation of Acute Tilted Amygdala Slices
  • 03:33Preparation of Coronal Amygdala Slices
  • 04:50Visualization and Stimulation of Presynaptic Fibers
  • 08:04Results: Optogenetic Activation of Long Range and Local Projections
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

Optogenetic approaches are widely used to manipulate neural activity and assess the consequences for brain function. Here, a technique is outlined that upon in vivo expression of the optical activator Channelrhodopsin, allows for ex vivo analysis of synaptic properties of specific long range and local neural connections in fear-related circuits.

Tags

Ex Vivo OptogeneticsNeural ProjectionsMicro-circuitsPatch-clamp RecordingsFear CircuitsChannel RhodopsinStereotactic Viral InjectionsVibratome SlicingAcute Brain SlicesInterface ChamberACSF

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53628?article_type=v

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
check_url/53628?article_type=v

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons
check_url/53628?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/53628?article_type=v

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
check_url/53628?article_type=v

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
check_url/53628?article_type=v

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
check_url/53628?article_type=v

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices
check_url/53628?article_type=v

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
check_url/53628?article_type=v

In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
check_url/53628?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Read Article