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Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
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JoVE Journal Neuroscience
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

DOI:
10.3791/58613-v

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08:58 min

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June 19, 2019

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, ,
1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine,Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS),University of Tsukuba, 3Faculty of Medicine,University of Tsukuba

Chapters

  • 00:04Title
  • 00:38Animal Preparation
  • 01:33Virus Injection
  • 02:47Electroencephalogram and Electromyogram (EEG/EMG) Electrode and Optical Fiber Implantation
  • 05:20EEG/EMG Monitoring
  • 07:19Results: Representative Effect of GABABNST Stimulation on State Transition in NREM and REM Sleep
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an example.

Tags

Keywords: Optogenetic ManipulationNeural CircuitsSleep/wakefulnessMiceNeuro CircuitSleep Wakefulness RegulationSurface EEGAnesthetized MouseStereotactic ApparatusAdeno-associated Virus VectorMicroinjectionBed Nucleus Of The Stria Terminalis (BNST)Electroencephalogram (EEG)Electromyogram (EMG)

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