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外来体的纯化和microRNA剖析来源于血液和文化传媒

JoVE Journal
Biologia

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JoVE Journal Biologia

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

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外来体的纯化和microRNA剖析来源于血液和文化传媒

DOI:

10.3791/50294-v

•

10:45 min

•

June 14, 2013

•

Marguerite K. McDonald, Kathryn E. Capasso, Seena K. Ajit

¹Department of Pharmacology & Physiology,Drexel University College of Medicine

Capítulos

00:05Título
01:37Exosome Purification from Blood
03:41miRNA Profiling of Exosomes from Blood Using Taqman Low Density Array (TLDA) Cards
08:38Results: Exosome RNA and microRNA Levels Offer a Unique Platform for Biomarker Discovery
10:16Conclusion

Summary

Tadução automática

存在稳定的microRNA（miRNA的）外来间通讯作为一种新型的模式产生了巨大的兴趣，为他们的潜在效用作为生物标志物和治疗干预的路由。在这里，我们展示了切体净化血液和文化传媒定量PCR确定的miRNA被运。

Tags

Exosomes MicroRNA MiRNA Profiling Purification Blood Cell Culture Media Biomarkers Gene Expression QPCR Taqman Low Density Array TEM Immunogold Labeling

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