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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
 

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Article DOI: 10.3791/50294-v • 10:45 min • June 14th, 2013
June 14th, 2013 •
1, 1, 1
1Department of Pharmacology & Physiology, Drexel University College of Medicine

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The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.

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Keywords: Exosomes MicroRNA MiRNA Profiling Purification Blood Cell Culture Media Biomarkers Gene Expression QPCR Taqman Low Density Array TEM Immunogold Labeling
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