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Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives,Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology,Columbia University , 3Department of Anesthesiology,Niigata University Graduate School of Medical and Dental Sciences
Capítulos
- 00:05Título
- 01:27Preparation of Equipment and Virus Particle Suspension
- 03:09Laminectomy
- 04:57Injection
- 06:21Wound Closure and Postoperative Care
- 07:35Results: Transfection of Dorsal Horn Neurons in the Mouse Spinal Cord
- 08:08Conclusion
Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.
