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Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl
 

Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAl

Article DOI: 10.3791/50375-v • 10:32 min • August 14th, 2013
August 14th, 2013 •
1,2,3,4, 1,2,3, 1,2,3, 1,2,3, 4, 1,2,3, 1,2,3
1U968, Institut National de la Santé et de la Recherche Médicale, 2UMR S 968, Université Pierre et Marie Curie, 3UMR 7210, Centre National de la Recherche Scientifique, 4Départment d'Ophtalmologie, Centre Hospitalier Universitaire de Bordeaux

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Resumo
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We used retinal samples from retinectomy for a transcriptomic analysis of retinal detachment. We developed a procedure that allows RNA conservation between the surgical blocks and the laboratory. We standardized a protocol to purify RNA by cesium chloride ultracentrifugation to assure that the purified RNAs are suitable for microarray analysis.

Tags

Retinal Detachment Neurosensory Retina Retinal Pigmented Epithelium Extracellular Fluid Vision Restoration Vitreous Gel Removal Surgical Specimens RNA Conservation Cesium Chloride Ultracentrifugation Global Gene Expression Analysis RT-PCR Microarray Analysis Inflammation
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