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Journal / Chemistry / Surface Passivation for Single-molecule Protein…
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Chemistry

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JoVE Journal Chemistry
Surface Passivation for Single-molecule Protein Studies
 

Surface Passivation for Single-molecule Protein Studies

Article DOI: 10.3791/50549-v • 10:35 min • April 24th, 2014
April 24th, 2014 •
1, 1, 2, 2, 2, 1
1Kavli Institute of NanoScience, Department of BioNanoScience, Delft University of Technology, 2Department of Chemistry, Seoul National University

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Resumo
Automatic Translation

We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.

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Keywords: Single-molecule Protein Surface Passivation Fluorescence Spectroscopy PEG Coating Surface Cleaning Surface Functionalization Piranha Etching Surface Immobilization
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