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Immunology and Infection

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JoVE Journal Immunology and Infection
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
 

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Article DOI: 10.3791/50599-v • 07:58 min • June 19th, 2013
June 19th, 2013 •
1,2, 2,3, 1,2,3, 2,3
1Department of Biology, Merrimack College, 2Center for Biotechnology and Biomedical Sciences, Merrimack College, 3Department of Technology R&D, Nexcelom Bioscience LLC

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Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.

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Image Cytometry Quantification Pathogenic Fungi Host Cells Cellular Pathogenesis Mechanisms Candida Albicans Histoplasma Capsulatum Cryptococcus Neoformans Infection Mammalian Hosts Macrophages Colony Forming Unit Analysis Flow Cytometry Disadvantages Limitations Fungal Species Wild-type Strains Viability Flow Cytometric Detection High Cost Biosafety Considerations Image-based Cytometric Methodology
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