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Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
DOI:

06:59 min

October 12, 2013

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Capítulos

  • 00:05Título
  • 00:58Isolate Mouse Colon Tissue
  • 02:07Obtain Human Colon Tissue and Denuding Epithelial Cells
  • 04:04Myofibroblast Isolation and Culture
  • 05:27Representative Primary Myofibroblast Culture Results
  • 06:37Conclusion

Summary

Tadução automática

The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.

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