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从小鼠和人类结肠组织初级肌成纤维细胞的分离

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

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从小鼠和人类结肠组织初级肌成纤维细胞的分离

DOI:

10.3791/50611-v

•

06:59 min

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October 12, 2013

•

Hassan Khalil, Wenxian Nie, Robert A Edwards, James Yoo

¹Department of Surgery,UCLA, ²Department of Pathology,UC Irvine

Capítulos

00:05Título
00:58Isolate Mouse Colon Tissue
02:07Obtain Human Colon Tissue and Denuding Epithelial Cells
04:04Myofibroblast Isolation and Culture
05:27Representative Primary Myofibroblast Culture Results
06:37Conclusion

Summary

Tadução automática

肌成纤维细胞是胃肠道，调节重要生理过程的正常和疾病状态的一个有影响力的基质细胞。我们描述了一种技术，它允许主肌纤维母细胞从小鼠和人结肠组织，它可以用于在体外实验的隔离。

Tags

Keywords: Primary Myofibroblasts Isolation Mouse Human Gastrointestinal Tract Stromal Cell Cell Culture Alpha-smooth Muscle Actin Vimentin Desmin

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