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打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

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打印温敏反向模具图案创作的双组分水凝胶的三维细胞培养

DOI:

10.3791/50632-v

•

10:49 min

•

July 10, 2013

•

Michael Müller, Jana Becher, Matthias Schnabelrauch, Marcy Zenobi-Wong

¹Department of Health Science & Technology,Cartilage Engineering & Regeneration, ²Biomaterials Department,Innovent e.V.

Capítulos

00:05Título
01:36Preparation of the Poloxamer 407 Solution
02:58Preparation of the 3D Printer
04:24Optimization of the Printing Parameters
06:42Printing and Elution of the Reverse Mold and Filling of the Voids
09:38Results: Patterned Two-component Hydrogel Printing
10:21Conclusion

Summary

Tadução automática

àbioprinter被用于创建图案的水凝胶的基础上牺牲的模具。泊洛沙姆模具与第二水凝胶回填，然后洗脱，留下空隙充满了三分之一的水凝胶。此方法使用快速的洗脱和良好的印刷泊洛沙姆产生复杂的生物大分子结构。

Tags

Keywords: Bioprinting Thermoresponsive Reverse Mold Two-component Hydrogel 3D Cell Culture Poloxamer Agarose Alginate Methacrylate FITC-fibrinogen Extrusion Bioprinting Sacrificial Mold Vascular Network Nerve Guide Conduit

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