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正向遗传途径<em>沙眼衣原体</em
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Forward Genetic Approaches in Chlamydia trachomatis
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正向遗传途径<em>沙眼衣原体</em

DOI:
10.3791/50636-v

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09:03 min

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October 23, 2013

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1Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis,Duke University Medical Center

Capítulos

  • 00:05Título
  • 01:20Chemical Mutagenesis
  • 03:49Clonal Isolation of Chlamydia trachomatis Mutant Strains by Plaque Purification
  • 07:14Representative Chlamydia Mutagenesis Results
  • 08:29Conclusion

Summary

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我们描述了一种方法来进行遗传分析化学诱变和全基因组测序的基础上衣原体 。此外，感染细胞内的DNA交换系统可用于遗传作图描述。此方法可广泛适用于微生物系统，缺乏改造系统和分子遗传工具。

Tags

Genetic AnalysisChlamydia TrachomatisForward GeneticsChemical MutagenesisWhole-genome SequencingGenotype-phenotype AssociationRecombinant StrainsGenetic ToolsIntractabilitySexually Transmitted DiseasesOcular Infections

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