/

/

Antibody Staining in C. Elegans Using "Freeze-Cracking"

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Antibody Staining in C. Elegans Using "Freeze-Cracking"

Antibody Staining in C. Elegans Using "Freeze-Cracking"

DOI:

10.3791/50664-v

•

13:10 min

•

October 14, 2013

•

Janet S. Duerr

¹Department of Biological Sciences,Ohio University

Capítulos

00:05Título
01:13Preparation of Frozen Nematode Slides
04:17Preparation of Slides of Individual Nematodes
05:32Fixation: Light Fix of Methanol-Acetone
06:30Fixation: Hard Fix Using Formaldehyde or Glutaraldehyde
08:22Preparation of Slides for Microscopy
10:32Representative Images
12:45Conclusion

Summary

Tadução automática

"Freeze-cracking," a method for exposing the inner tissues of the nematode C. elegans to antibodies for protein localization, is demonstrated.

Tags

Antibody Staining C. Elegans Freeze-cracking Cuticle Nematodes Mechanical Permeabilization Fixatives Sample Recovery Morphology Optimization Chemical Permeabilization

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Generation of Stable Transgenic C. elegans Using Microinjection

Method for Whole Mount Antibody Staining in Chick

Generation of Transgenic C. elegans by Biolistic Transformation

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

C. elegans Chemotaxis Assay

A Method for Culturing Embryonic C. elegans Cells

An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans

Methods for Skin Wounding and Assays for Wound Responses in C. elegans

Read Article