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Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
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Capítulos
- 00:05Título
- 02:22Photolithography Fabrication of Photoresist Master Mold
- 03:10Soft Lithography Fabrication of PDMS Microchannel Network
- 04:34HUVEC Culture and Seeding in the Chip
- 07:03Cell Staining and Microscope Characterization
- 08:10Results: Multi-depth Microchannel Networks Mimic the Complex 3D Geometries of In vivo Microvessel
- 09:35Conclusion
A microchannels-on-a-chip platform was developed by the combination of photolithographic reflowable photoresist technique, soft lithography, and microfluidics. The endothelialized microchannels platform mimics the three-dimensional (3D) geometry of in vivo microvessels, runs under controlled continuous perfusion flow, allows for high-quality and real-time imaging and can be applied for microvascular research.
