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使用点击化学测量的影响，病毒感染宿主细胞RNA合成
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
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使用点击化学测量的影响，病毒感染宿主细胞RNA合成

DOI:
10.3791/50809-v

•

09:35 min

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August 09, 2013

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, , , ,
1Department of Pathology, Sealy Center for Vaccine Development, Center for Biodefense and Emerging Infectious Diseases,University of Texas Medical Branch

Capítulos

  • 00:05Título
  • 01:16Analysis of Transcription by Fluorescence Microscopy
  • 04:16Analysis of Transcription by Flow Cytometry
  • 06:08Infecting 293 Cells with MP-12
  • 08:52Conclusion

Summary

Tadução automática

Tadução automática

这个方法描述了使用的点击化学感染裂谷热病毒（RVFV）MP-12菌株后，在宿主细胞的转录变化来衡量。结果可以可视化，通过荧光显微镜定性或定量地获得通过流式细胞仪。此方法是适应性强，用于与其他病毒。

Tags

Keywords: Click ChemistryViral InfectionHost-cell RNA SynthesisTranscriptional SuppressionRift Valley Fever Virus5-ethynyluridineFluorescent AzideFlow CytometryFluorescence Microscopy

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