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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
 

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

Article DOI: 10.3791/50823-v • 14:14 min • January 6th, 2014
January 6th, 2014 •
1,2,3, 1,3, 3, 1,3
1Department of Pediatrics, Harvard Medical School, 2Department of Pediatric Gastroenterology, MGH for Children, 3Mucosal Immunology and Biology Research Center, Massachusetts General Hospital

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Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.

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In Vitro Coculture Assay Pathogen Induced Neutrophil Trans-epithelial Migration Mucosal Surfaces Innate Immune Responses Inflammatory Cells Destructive Tissues In Vitro Models Molecular Mechanisms Pathogenic Infection Polarized Epithelial Monolayers Transwell Filters Basolateral To Apical Migration Lung Epithelial Cells Neutrophil Isolation PAO1 K12 E. Coli
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