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芯片上的压力诱导抗生素易感性测试

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Stress-induced Antibiotic Susceptibility Testing on a Chip

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芯片上的压力诱导抗生素易感性测试

DOI:

10.3791/50828-v

•

12:41 min

•

January 08, 2014

•

Maxim Kalashnikov, Jennifer Campbell, Jean C. Lee, Andre Sharon³, Alexis F. Sauer-Budge⁴

¹Fraunhofer USA Center for Manufacturing Innovation, ²Division of Infectious Diseases, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, ³Department of Mechanical Engineering,Boston University, ⁴Department of Biomedical Engineering,Boston University

Capítulos

00:05Título
01:40Making the Poly(dimethylsiloxane) (PDMS) Layer
02:29Assembling the Flow Cell
03:41Loading the Bacterial Suspension
05:08Loading the Solutions
06:15Image Acquisition
08:02Disinfecting the Flow Cell and Counting the Bacteria
09:21Results: Representative Bacterial Susceptibility Imaging and Survival Data
12:07Conclusion

Summary

Tadução automática

我们开发了一个用于快速抗生素易感性检测的微流体平台。流体以高速通过在微流体通道底部固定的细菌。在压力和抗生素的存在下，易感菌株会迅速死亡。然而，耐药细菌可以在这些紧张的条件下存活下来。

Tags

Microfluidics Antibiotic Susceptibility Testing Stress-based Testing Bacterial Culture PDMS Cell Viability Fluorescence Image Processing Mechanical Stress Enzymes Microarray Substrates Drug Development Bacterial Suspension Experiments

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