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JoVE Journal Neuroscience
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
 

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

Article DOI: 10.3791/50905-v • 10:51 min • November 25th, 2013
November 25th, 2013 •
*1, *1, 2, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
* These authors contributed equally

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Resumo
Automatic Translation

Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

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Keywords: Subventricular Zone Neurogenic Niche Neuroblast Migration Rostral Migratory Stream Olfactory Bulb In Vivo Electroporation Time-lapse Imaging Acute Brain Slices Neurogenesis Brain Repair
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