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盲肠结扎穿孔致脓毒症作为模型小鼠中自噬研究

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

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盲肠结扎穿孔致脓毒症作为模型小鼠中自噬研究

DOI:

10.3791/51066-v

•

06:40 min

•

February 09, 2014

•

Ilias I. Siempos³, Hilaire C. Lam, Yan Ding, Mary E. Choi, Augustine M. K. Choi, Stefan W. Ryter

¹Department of Medicine, Division of Pulmonary and Critical Care Medicine,Brigham and Women’s Hospital, ²Department of Medicine, Renal Division,Brigham and Women’s Hospital, Harvard Medical School, ³First Department of Critical Care Medicine and Pulmonary Services,University of Athens Medical School, Evangelismos Hospital, Athens, Greece

Capítulos

00:05Título
01:45Cecal Ligation and Puncture
03:44Tissue harvest and Fixation
05:06Results: Autophagy in Mice after Cecal Ligation and Puncture
06:05Conclusion

Summary

Tadução automática

实验性败血症可以在小鼠体内利用盲肠结扎穿孔（CLP）方法来诱导。当前的协议，以评估在CLP诱导的脓毒症的上下文中的自噬体内这里提出：一个协议，用于使用（GFP）-LC3的小鼠测定自噬和协议，用于测量自噬体形成通过电子显微镜。

Tags

Keywords: Cecal Ligation And Puncture Sepsis Autophagy GFP-LC3 Electron Microscopy Critical Care Medicine

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