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隔离与人类心室肌细胞的功能研究从新鲜手术样品
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Medicina
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JoVE Journal Medicina
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
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隔离与人类心室肌细胞的功能研究从新鲜手术样品

DOI:
10.3791/51116-v

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14:39 min

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April 21, 2014

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Capítulos

  • 00:05Título
  • 02:47Collection and Processing of Myocardial Samples
  • 03:52Washing and Digestion of Myocardial Chunks
  • 06:43Cell Resuspension and Ca2+ Readapation
  • 08:28Functional Evaluation of Isolated Cardiomyocytes
  • 11:11Resultados
  • 13:16Conclusion

Summary

Tadução automática

Tadução automática

心脏疾病的细胞基础上的现有知识大多依赖于对动物模型的研究。在这里，我们描述和验证了一种新的方法从人心肌小型手术样品得到单一可行的心肌细胞。人心肌细胞可用于电生理学研究和药物测试。

Tags

CardiomyocytesVentricular MyocardiumCardiac SurgeryElectrophysiologyIntracellular CalciumCardiac DiseaseCellular And Molecular BasisTherapeutic TargetsTranslational Value

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