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通过激光捕获微分路,从小鼠脊髓运动神经细胞体中生成RNA进行转录分析
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
DOI:

11:46 min

January 13, 2014

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Capítulos

  • 00:05Título
  • 02:23Preparing Spinal Cord Sections
  • 04:21Staining Spinal Cord Sections
  • 07:20Laser Capture Microdissection (LMD)
  • 10:08Results: Motor Neuron Isolation
  • 11:07Conclusion

Summary

Tadução automática

通过激光捕捉微分,用 Azure B 染色 70% 乙醇的脊髓部分后,从小鼠脊髓运动神经元的细胞体中制备了高质量的总RNA。从 3,000-4,000 个运动神经元中恢复足够的 RNA(+40-60 ng),以便通过 RNA-seq 和 qRT-PCR 进行下游 RNA 分析。

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