/
/
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 02:22Microfluidic Device Fabrication
- 04:19Microfluidic Assay Preparation
- 06:11Sample Preparation: Human Neutrophils From Capillary Blood
- 06:49Microscopy and Image Analysis for Neutrophil Chemotaxis Measurements
- 07:53Results: Neutrophils Chemotaxis Assay From Whole Blood
- 08:55Conclusion
This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.
