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三维文化：一种工具来研究正常腺泡结构与恶性转化的乳腺细胞
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JoVE Journal Medicina
Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
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三维文化：一种工具来研究正常腺泡结构与恶性转化的乳腺细胞

DOI:
10.3791/51311-v

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08:33 min

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April 25, 2014

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,
1Department of Internal Medicine,University of Michigan Comprehensive Cancer Center, 2Department of Pathology,University of Michigan Comprehensive Cancer Center

Capítulos

  • 00:05Título
  • 01:57Three Dimensional Culture Preparation
  • 03:34Quantification of the Malignant vs. Benign Breast Phenotype
  • 04:31Immunofluorescence Staining of Acinar Structures Grown on Matrigel
  • 06:36Results: Comparison of Benign vs. Malignant Phenotypes
  • 08:05Conclusion

Summary

Tadução automática

Tadução automática

对重组基底膜乳腺上皮细胞的三维培养是扼要重述在体内的体系结构的良性乳房，并从良性乳腺表型分化的恶性表型的有效方法。重要的是，该系统可以应用到研究浸润性癌中的其他组织中。

Tags

3D CulturesAcinar ArchitectureBreast CellsMalignant TransformationExtracellular MatrixBenign And Malignant Breast CellsPolarityCell ProliferationApoptosisMolecular Markers

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