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Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
 

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis

Article DOI: 10.3791/51312-v • 08:12 min • September 1st, 2014
September 1st, 2014 •
1, 1, 2, 1, 1, 1, 1, 1, 1, 1, 2
1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH

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The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

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Endothelial Cell Tube Formation Assay In Vitro Study Angiogenesis Protocol Primary Endothelial Cells Immortalized Endothelial Cells Conditioned Media Basement Membrane Matrix Capillary-like Structures Angiogenic Signals Phase Contrast Inverted Microscope Calcein AM Fluorescence Microscopy Confocal Microscopy Branch Sites/nodes Loops/meshes Number Of Tubes Formed Genes And Pathways
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