Journal
/
Bioengenharia
/
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
JoVE Journal
Bioengenharia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

DOI:
10.3791/51321-v

•

05:48 min

•

March 18, 2014

•

,
1Division of Cancer Research, Ninewells Hospital and Medical School,University of Dundee, 2Institute of Medical Biology,A*Star, Singapore

Capítulos

  • 00:05Título
  • 01:20In Vitro Construction of Fibroblast-derived Native Matrix
  • 03:01Invasion Assay with Tumor Squamous Cell Carcinoma (SCC) Keratinocytes
  • 04:47Results: Representative cSCC Invasion into the Native Matrix
  • 05:24Conclusion

Summary

Tadução automática

Tadução automática

Tissue engineered fibroblast-derived native matrix is an emerging tool to generate a stromal substrate which supports epithelial cell proliferation and differentiation. Here a protocol applying this methodology to assess the impact of different stromal cell types on tumor cell biology is presented.

Tags

Tumor Stromal Microenvironment3D Organotypic CulturesExtracellular MatrixType I CollagenEngelbreth-Holm-Swarm Mouse SarcomaDermal FibroblastsRecessive Dystrophic Epidermolysis BullosaNative MatrixEpithelial-mesenchymal Cell InteractionsType VII CollagenTumor Cell Invasion

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/51321?article_type=v

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
check_url/pt/51321?article_type=v

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
check_url/pt/51321?article_type=v

Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
check_url/pt/51321?article_type=v

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
check_url/pt/51321?article_type=v

Tissue Engineering of a Human 3D in vitro Tumor Test System
check_url/pt/51321?article_type=v

Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
check_url/pt/51321?article_type=v

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
check_url/pt/51321?article_type=v

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
check_url/pt/51321?article_type=v

Sandwich-like Microenvironments to Harness Cell/Material Interactions
check_url/pt/51321?article_type=v

In Vitro Model of Physiological and Pathological Blood Flow with Application to Investigations of Vascular Cell Remodeling

Read Article