Journal
/
Química
/
Nucleoside Triphosphates – From Synthesis to Biochemical Characterization
JoVE Journal
Química
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Química
Nucleoside Triphosphates – From Synthesis to Biochemical Characterization

Nucleoside Triphosphates – From Synthesis to Biochemical Characterization

DOI:
10.3791/51385-v

•

15:22 min

•

April 03, 2014

•

, ,
1Department of Chemistry and Biochemistry,University of Bern

Capítulos

  • 00:05Título
  • 02:30Synthesis of the Modified Nucleoside Triphosphates
  • 05:56HPLC Purification of the Modified Nucleoside Triphosphates
  • 07:40Primer Extension Reactions and TdT Polymerizations
  • 11:05Results: Biochemical Characterization of Nucleoside Triphosphates
  • 14:02Conclusion

Summary

Tadução automática

Tadução automática

The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.

Tags

Nucleoside TriphosphatesPhosphoramidite PrecursorsSolid-phase SynthesisModified Nucleic AcidsFunctional TaggingRibozymesDNAzymesPhosphorous(III)-based ReagentsPrimer Extension ReactionsTdT Tailing PolymerizationChemical Biology

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/51385?article_type=v

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
check_url/pt/51385?article_type=v

Synthesis and Characterization of Functionalized Metal-organic Frameworks
check_url/pt/51385?article_type=v

Metal-free Synthesis of Ynones from Acyl Chlorides and Potassium Alkynyltrifluoroborate Salts
check_url/pt/51385?article_type=v

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
check_url/pt/51385?article_type=v

Synthesis, Characterization, and Functionalization of Hybrid Au/CdS and Au/ZnS Core/Shell Nanoparticles
check_url/pt/51385?article_type=v

Synthesis and Characterization of Supramolecular Colloids
check_url/pt/51385?article_type=v

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
check_url/pt/51385?article_type=v

Synthesis and Characterization of Fe-doped Aluminosilicate Nanotubes with Enhanced Electron Conductive Properties
check_url/pt/51385?article_type=v

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
check_url/pt/51385?article_type=v

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Read Article