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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
 

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Article DOI: 10.3791/51385-v • 15:22 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 1
1Department of Chemistry and Biochemistry, University of Bern

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Resumo
Automatic Translation

The protocol described herein aims to explain and abridge the numerous obstacles in the way of the intricate route leading to modified nucleoside triphosphates. Consequently, this protocol facilitates both the synthesis of these activated building-blocks and their availability for practical applications.

Tags

Nucleoside Triphosphates Phosphoramidite Precursors Solid-phase Synthesis Modified Nucleic Acids Functional Tagging Ribozymes DNAzymes Phosphorous(III)-based Reagents Primer Extension Reactions TdT Tailing Polymerization Chemical Biology
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