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HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
 

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Article DOI: 10.3791/54124-v • 11:29 min • July 20th, 2016
July 20th, 2016 •
1, 1
1Department of Medical Sciences, Human Genetics Foundation (HuGeF), University of Turin

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The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.

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HPLC-based Assay Extracellular Nucleotide/nucleoside Metabolism Chronic Lymphocytic Leukemia Cells Reverse Phase High Performance Liquid Chromatography Quantifying Purine Metabolism Adenosine Production Cytoprotective Molecule Immunosuppressive Molecule Tumor Microenvironment Easy And Reproducible Measurement Purified CLL Lymphocytes Purifying Peripheral Blood Mononuclear Cells Density Gradient Centrifugation
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