Journal
/
/
HPLC 기반 분석은 인간의 만성 림프 구성 백혈병 세포에 세포 외 뉴클레오티드 / 뉴 클레오 시드 신진 대사를 모니터링하기
JoVE Journal
Química
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Química
HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
DOI:

11:29 min

July 20, 2016

,

Capítulos

  • 00:05Título
  • 00:59Isolation of Leukemic Lymphocytes from Blood Samples of CCL Patients
  • 02:09Purification of Leukemic B Cells by Negative Isolation
  • 03:27Program the HPLC Method
  • 05:07Pretreatment with the Inhibitors and Incubation with the Substrate (AMP)
  • 06:16Samples Preparation for HPLC and HPLC Measurements of Purines
  • 08:14Results: Quantification of Purine Compounds in Freshly Purified CLL Cells from a Representative CD73+ Patient
  • 09:36Conclusion

Summary

Tadução automática

The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions.

Vídeos Relacionados

Read Article