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Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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Capítulos
- 00:05Título
- 01:50Prepare Tumor Cells and Ear Injection
- 03:36Ear Surgery
- 04:40Immunofluorescence Staining and Intravital Imaging with a Stereomicroscope
- 07:06Results: Intravital Immunofluorescence Imaging
- 08:26Conclusion
The extracellular matrix undergoes substantial remodeling during wound healing, inflammation and tumorigenesis. We present a novel intravital immunofluorescence microscopy approach to visualize the dynamics of fibrillar as well as mesh-like matrix components with high spatial and temporal resolution using epifluorescence or two-photon microscopy.
