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Imunologia e Infecção
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Hurtig screening af HIV revers transkriptase og Integrase hæmmere
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

Hurtig screening af HIV revers transkriptase og Integrase hæmmere

DOI:
10.3791/51400-v

•

05:46 min

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April 09, 2014

•

,
1HIV Drug Resistance Program,National Cancer Institute

Capítulos

  • 00:05Título
  • 01:09Viral Stock Generation
  • 02:29Compound Screening
  • 03:42Measurement of Luciferase Activity
  • 04:41Results: Representative Luciferase Signal Readout Inhibition
  • 05:22Conclusion

Summary

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Tadução automática

Her beskriver vi cellulær cytotoksicitet og enkelt runde infektivitet analyser, som giver mulighed for hurtig og præcis screening af forbindelser for at bestemme deres cellulære cytotoksicitet (CC 50) og IC50-værdier mod WT og resistent HIV-1 lægemiddel.

Tags

HIV Reverse TranscriptaseHIV IntegraseAntiviral CompoundsDrug ResistanceCytotoxicity AssayLuciferase ReporterViral InfectivityScreening PlatformHigh-throughput Screening

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