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蛋白通过流体动力基因传递在小鼠体内瞬时表达

JoVE Journal
Biologia

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JoVE Journal Biologia

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Please note that all translations are automatically generated. Click here for the English version.

蛋白通过流体动力基因传递在小鼠体内瞬时表达

DOI:

10.3791/51481-v

•

12:54 min

•

May 05, 2014

•

Daniella Kovacsics, Jayne Raper

¹Department of Biological Sciences,Hunter College, CUNY

Capítulos

00:05Título
01:23Weighing Mice and Preparing Syringes
03:38Hydrodynamic DNA Delivery
08:18Large-volume Tail Vein Injection using an Alternative Hand Position
09:41Representative Analysis of Transgene Expression
11:50Conclusion

Summary

Tadução automática

在由流体动力基因递送裸DNA的体内转染引入基因到最小的炎症反应的动物的组织中。产生基因产物的足够量，使得基因功能和调节以及蛋白质结构和功能可以被分析。

Tags

Keywords: Hydrodynamic Gene Delivery In Vivo Gene Expression Transgene Delivery Plasmid DNA Transient Protein Expression Apolipoprotein L-I Haptoglobin-related Protein Rodent Models

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