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两种方法从子宫切除标本小学建立人子宫内膜基质细胞

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Medicina

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JoVE Journal Medicina

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens

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两种方法从子宫切除标本小学建立人子宫内膜基质细胞

DOI:

10.3791/51513-v

•

09:15 min

•

May 23, 2014

•

Kasey Jividen*¹, Mercedeh Javanbakht Movassagh*¹, Amir Jazaeri, Hui Li

¹Department of Pathology,University of Virginia, ²Department of Obstetrics & Gynecology,University of Virginia

Capítulos

00:05Título
01:34Prepariation of Primary Cell Lines using the Scraping Method
04:56Preparation of Primary Cell Lines using the Trypsin Method
07:07Results: Establishment of an Endometrial Stromal Culture
08:53Conclusion

Summary

Tadução automática

建立基层子宫内膜间质细胞培养系统从子宫切除标本是追求研究的目的繁多前一个有价值的生物技术和关键的一步。在这里，我们描述了两种方法用于建立从人类患者手术切除的子宫内膜组织间质文化。

Tags

Primary Human Endometrial Stromal Cells In Vitro Cell Culture Hysterectomy Specimens Scraping Method Trypsin Method Endometrial Pathologies Gynecological Cancers Infectious Diseases Reproductive Deficiencies Quantitative Real-time PCR Immunofluorescence

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