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在实时的间皮细胞卵巢癌球体附件和入侵评估

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

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在实时的间皮细胞卵巢癌球体附件和入侵评估

DOI:

10.3791/51655-v

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14:25 min

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May 20, 2014

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Maree Bilandzic, Kaye L. Stenvers²

¹Reproductive Development and Cancer Laboratory,MIMR-PHI Institute of Medical Research, ²Department of Developmental Biology and Anatomy,Monash University

Capítulos

00:05Título
02:05Optional Fluorescent Cell Labeling Method
03:24Generation of Spheroids in Methylcellulose
04:28Real Time Cell Analyzer (RTCA) Cell Invasion Assay
05:46Programming the RTCA Instrument
07:25Generation of LP9 Monolayer and Measurement of Spheroid Invasion
09:49Results: Real Time Assessment of Ovarian Cancer Spheroid Invasion
13:42Conclusion

Summary

Tadução automática

卵巢癌细胞侵入的腹膜间皮衬里是一个动态过程，随着时间的推移。利用实时分析器，卵巢癌细胞中的球状体，间皮细胞共培养模型的侵袭能力可以量化在延长的时间段，提供见解因子调控的转移过程。

Tags

Keywords: Ovarian Cancer Spheroid Mesothelial Cells Invasion Real-time Analysis Metastasis Peritoneal Environment Extracellular Matrix Cell Lines Quantitative Measurements

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