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Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

DOI:
10.3791/51678-v

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09:04 min

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July 09, 2014

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, ,
1Department of Medicine,Roswell Park Cancer Institute, 2Department of Medicine, School of Medicine and Biomedical Sciences,University of Buffalo

Capítulos

  • 00:05Título
  • 01:08Labeling Macrophages In Vivo and Bronchoalveolar Lavage Harvesting
  • 03:58Culturing and Harvesting Fungus
  • 05:13Challenging Macrophages with Conidia
  • 07:26NADPH Oxidase in Conidia Phagocytosis
  • 08:42Conclusion

Summary

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A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy.

Tags

Alveolar MacrophagesAspergillus FumigatusConfocal MicroscopyNADPH OxidaseAnti-fungal ActivityPhagocytosisReactive Oxygen Intermediates (ROIs)Host Defense

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