É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:29Dissection of Mouse Mesencephalon
- 04:40Cell Dissociation
- 06:37Cell Plating
- 07:29Immunofluorescence Protocol
- 09:20Results: Primary Culture of Mouse Dopaminergic Neurons
- 10:49Conclusion
Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.
