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Imunologia e Infecção
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Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

DOI:
10.3791/51780-v

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07:20 min

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August 08, 2014

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1Department of Laboratory Medicine & Pathology, Center for Immunology,University of Minnesota

Capítulos

  • 00:05Título
  • 01:24Stromal Cell Isolation from Mouse Thymus
  • 03:20TEC Identification, Panning, and Purification
  • 04:48Results: Representative Thymic Epithelial Populations
  • 06:44Conclusion

Summary

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Here we describe an efficient method for isolation, identification, and purification of mouse thymic epithelial cells (TECs). The protocol can be utilized for studies of thymus function for normal T cell development, thymus dysfunction, and T cell reconstitution.

Tags

Thymic Epithelial CellsThymusT Cell DevelopmentCell IsolationCell PurificationCell CharacterizationFlow CytometryCell DepletionCell Sorting

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