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La coltivazione di cellule di mammifero utilizzando un singolo-uso del sistema pneumatico Bioreattore
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
DOI:

11:42 min

October 10, 2014

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Capítulos

  • 00:05Título
  • 01:35Login and Calibration
  • 03:41Autoclave, Sensor, and Reagent Vessel Installation
  • 06:43Medium- and Micro-carriers Addition, Equilibration, and One-point DO Calibration
  • 08:28Run Initiation, Cell Inoculation, and Sampling
  • 10:11Results: Characterization and Analysis of Bioreactor Cell Culture and Viral Growth
  • 11:12Conclusion

Summary

Tadução automática

Using a pneumatic bioreactor, we demonstrate the assembly, operation, and performance of this single-use bioreactor system for the growth of mammalian cells.

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