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Immunology and Infection
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JoVE Journal Immunology and Infection
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
 

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Article DOI: 10.3791/52222-v • 07:31 min • November 1st, 2014
November 1st, 2014 •
1, 2, 2
1Biological Department, King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield

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The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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Keywords: Equine IgE FcεRI In Vitro Model RBL-2H3.1 Cells Transfection Allergic Response β-hexosaminidase Release Diagnostic Tool Therapeutic Intervention
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