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Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
 

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Article DOI: 10.3791/52240-v • 12:21 min • December 13th, 2014
December 13th, 2014 •
1, 1
1Department of Biology, Baylor University

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The steady state level of specific mRNAs is determined by the rate of synthesis and decay of the mRNA. Genome-wide mRNA degradation rates or the decay rates of specific mRNAs can be measured by determining mRNA half-lives. This protocol focuses on measurement of mRNA decay rates in Saccharomyces cerevisiae.

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MRNA Decay Rates Saccharomyces Cerevisiae Rpb1-1 Strain Steady State Levels MRNA Regulation Transcription Inhibition Monitoring MRNA Disappearance Quantifying Decay Rate MRNA Half-life Determination RNA Polymerase II Temperature Sensitive Allele Transcriptional Inhibitors Regulatable Promoter Galactose Inducible Promoter TET-off System Measurement Technique
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